Journal: Molecular Therapy. Nucleic Acids
Article Title: Functional Significance and Therapeutic Potential of miRNA-20b-5p in Esophageal Squamous Cell Carcinoma
doi: 10.1016/j.omtn.2020.05.015
Figure Lengend Snippet: DNA Methylation Status of miR-20b-5p by MassARRAY (A) Methylation profile of CpG sites for the miR-20b-5p gene. The color of the circles is related to the percentage of methylation at each CpG site. Boxes indicate the different methylation patterns between 14 ESCC samples and the corresponding tissues. (B) Hierarchical cluster analysis of CpG site methylation profiles of the miR-20b-5p promoter region in esophageal squamous cell carcinoma (n = 14) and control esophageal tumors (n = 14). The color gradient between green and red indicates methylation of each miR-20b-5p unit in each sample ranging from 0% to 60%. Gray represents technically inadequate or missing data. (C) Evaluation of CpG methylation within the miR-20b-5p promoter. The distribution of the seven analyzed CpG units within miR-20b-5p is shown. Data are presented as the mean value ± SD from triplicate experiments. @@ p < 0.01.
Article Snippet: In addition, the mass spectra data were obtained with MassARRAY compact matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) (Agena Bioscience, USA), while the methylation status of the individual units were evaluated with EpiTYPER 1.0.5 (Agena Bioscience, USA).
Techniques: DNA Methylation Assay, Methylation, Control, CpG Methylation Assay